Coeliac disease

Clinical information

Coeliac disease is a systemic autoimmune disease with a pronounced genetic predisposition which may affect different organ systems. The prevalence of the disease is estimated to amount to approximately 1%, although experts suppose there are a large number of additional cases which are not diagnosed due to “atypical” or mild symptoms. Coeliac disease is triggered by the consumption of gluten, which makes up about 90% of the protein contents of many cereal grains. In most cases, the disease manifests as severe inflammation and damage to the mucous membrane of the small intestine (enteropathy). In conjunction with the resulting disturbance of nutrient absorption, a wide range of clinical gastrointestinal and non-gastrointestinal symptoms can develop (including chronic diarrhoea, vomiting, abdominal pain, cramps, short stature, weight loss, delayed puberty, spontaneous abortions, anaemia and osteoporosis). Furthermore, dermatitis herpetiformis (Duhring’s disease), a chronic skin rash, can occur.

Both genetic and environmental factors contribute to the development of coeliac disease. Enteropathy, which is characteristic of coeliac disease, is caused by an overreaction of the immune system to gluten components, especially the so-called gliadin. Gliadin is only partially digested in the small intestine. If there are gaps in the intestinal epithelium, as is typical in patients with coeliac disease, the resulting gliadin fragments (peptides, consisting of 33 amino acids, 33-mer) can pass through the intestinal barrier and reach the connective tissue underneath. There, the enzyme tissue transglutaminase (tTG) modifies (deamidates) the amino acid glutamine (Q) into the amino acid glutamate (E) at certain sites of the gliadin peptides. This modification brings on an immunological reaction in the case of genetic predisposition. The activation of B cells leads to the production of antibodies against the deamidated gliadin peptides and against the body’s own tissue transglutaminase. In addition, the T cells secrete pro-inflammatory cytokines which cause an inflammatory reaction in the tissue. The immunological overreaction and the inflammation of the epithelium of the small intestine lead to apoptosis of the enterocytes, atrophy of the villi and widening of the intestinal crypts (hyperplasia). These damages mean that the intestinal mucosa is no longer able to absorb sufficient nutrients from the digested food and to transport them into the bloodstream.

Diagnostics

Monkey liver: Aab against endomysiumAccording to the guidelines of the European Society of Pediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) (Husby et al., 2020), patients with relevant symptoms should first be tested for anti-tTG antibodies (IgA) and total IgA antibodies, as these antibodies are particularly specific and sensitive. If the anti-tGG IgA is more than ten times the antibody concentration at the upper limit of normal (> 10x ULN) and a sample taken later is positive for anti-endomysium (EmA) IgA, a biopsy, which is otherwise required to confirm the diagnosis, can be omitted.The guidelines also emphasise the usefulness of coeliac-specific IgG-based tests in other cases, such as tests for the detection of antibodies against deamidated gliadin peptides (DGP). If there is a general IgA deficiency – as often observed in coeliac disease patients – anti-DGP antibodies (IgG) are considered an important alternative indicator of coeliac disease. EmA, which targets tTG as antigen, can be detected in IIFT on tissue sections of primate liver, primate oesophagus or primate intestine.

EUROIMMUN offers different ELISA, EUROLINE, ChLIA and IIFT test systems for the detection of antibodies against tissue transglutaminase, endomysium as well as against deamidated gliadins.

Techniques

Method
Parameter
Substrate
Species
EUROLINE
Autoimmune Gastrointestinal Diseases IgA
(tissue transglutaminase (endomysium), gliadin-analogue
fusion peptide (GAF-3X), mannan (ASCA))
EUROLINE
EUROLINE
Autoimmune Gastrointestinal Diseases IgG
(tissue transglutaminase (endomysium),
gliadin-analogue fusion peptide (GAF-3X),
parietal cell antigen (PCA) separately
Intrinsic factor, mannan (ASCA))
EUROLINE
EUROLINE
positive control serum: IgA, human, 100x concentrated
for DL 1910 A
EUROLINE
positive control serum: IgG, human, 100x concentrated
for DL 1910 G
ELISA
tissue transglutaminase
(endomysium)
antigen-coated
microplate wells
ELISA
tissue transglutaminase
(endomysium)
antigen-coated
microplate wells
EUROLINE
Coeliac Disease Profile
(tissue transglutaminase (endomysium),
gliadin-analogue fusion peptide (GAF-3X) separately)
EUROLINE
IIFT
antibodies against endomysium
(EMA IgA control)
IIFT
antibodies against endomysium
(EMA IgG control)
ChLIA
IDS CELIAC Control Set 1
3 x 1.5mL Control 1/2
ChLIA
IDS t-TG IgG Control Set 1
3 x 1.5mL Control 1/2
ChLIA
IDS t-TG IgA 1
antigenic coated magnetic particles
ChLIA
IDS t-TG IgG 1
antigenic coated magnetic particles
IIFT
endomysium
small intestine
monkey
IIFT
antibodies against endomysium plus gliadin (GAF-3X)
(EMA plus gliadin GAF-3X IgA control)
IIFT
antibodies against endomysium plus gliadin (GAF-3X)
(EMA plus gliadin GAF-3X IgG control)
IIFT
EUROPLUS
endomysium
gliadin (GAF-3X)
2 BIOCHIPs per field:
small intestine
gliadin (GAF-3X) BIOCHIPs

monkey
IIFT
endomysium
liver
monkey
IIFT
Coeliac Disease Screen (LM) EUROPattern
endomysium
liver
monkey
IIFT
EUROPLUS
endomysium
gliadin (GAF-3X)
2 BIOCHIPs per field:
liver
gliadin (GAF-3X) BIOCHIPs

monkey
IIFT
endomysium
endomysium
liver
small intestine
(2 BIOCHIPs per field)
monkey
monkey
ChLIA
Anti-Gliadin (GAF-3X) ChLIA (IgG)
Antigen-coated magnetic particles
ChLIA
Anti-Gliadin (GAF-3X) ChLIA (IgA)
Antigen-coated magnetic particles
ChLIA
Control set Anti-Gliadin (GAF-3X)-ChLIA (IgG)
2 x 0.5 ml control 1/2
ELISA
gliadin (GAF-3X)
antigen-coated
microplate wells
ChLIA
IDS Deamidated Gliadin IgA 1
antigenic coated magnetic particles
ChLIA
IDS Deamidated Gliadin IgG 1
antigenic coated magnetic particles
1Product manufactured by third party. Please contact your local EUROIMMUN representative for more information.
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